Laboratory Medicine Program

Inulin clearance (Research only)

Clinical Decription:
Inulin is not metabolized nor is it absorbed or secreted by the renal tubules. Thus, the quantitation of serum or urine inulin and the calculation of inulin clearance allows the measurement of the glomerular filtration rate. Serum and urine samples are deproteinized with cadmium sulfate and sodium hydroxide. Inulin in the deproteinized sample reacts with anthron dissolved in concentrated sulfuric acid top form a blue-green chromogen which is measured at 603 nm.
<br/><em>Davidson WD, Sackner MA. J Lab Clin Med, 1963;62(2):351-6 White RP, Samson FE, J Lab Clin Med, 1954;43(3):475-78</em>

Method: Colorimetric; Colourimetric microplate

Component Tests Used: n/a

Reference Ranges Used:
20-29y Female 84-156 mL/min Male 90-174 mL/min 30-39y Female 82-150 mL/min Male 88-168 mL/min 40-49y Female 82-146 mL/min Male 78-162 mL/min 50-59y Female 66-142 mL/min Male 68-152 mL/min

Specimen Type: lesional tissue
Volume: 10 urine or serum mL (minimum: 10 urine or serum mL)

Shipping: Sample on dry ice

Special Instructions: Add 10 mg thymol/100 mL urine. Record volume and aliquot urine. Centrifuge and aliquot blood samples.

Testing Schedule(s): on request, but must be a minimum of 25 samples, 4 week prior arrangement.

Turnaround Time: 20 days

For more information, call 416.340.5227 or 1.866.865.5227