Laboratory Medicine Program

Hemoglobin electrophoresis

Clinical Decription:
This test should be ordered if a hemoglobinopathy or thalassemia are being considered. Test is performed by HPLC (High Performance Liquid Chromatography) which results in a separation of hemoglobin subtypes and an accurate assessment of Hb A2 (for B thal diagnosis). Also included if appropriate is a Hb H preparation to exclude alpha thalassemia. A CBC and ferritin result should be included to assist in the interpretation of results.
<br/><em>Bah BJ, Hemoglobinopathy Diagnosis. Blackwell Science , United Kindom, 2001:20.</em>

Method: Capillary Electrophoresis; Sebia Capillarys 2 Flex

Component Tests Used: n/a

Reference Ranges Used:
Adult HbA ;0.965 - 0.98HbA2 ; 0.02 - 0.035Hb F;<= 0.03 HbA2 in presence of HbS trait; 0.025-0.050 HbA2 in presence of HbS disease; 0.025-0.055

Specimen Type: whole blood -EDTA tube
Collected In: EDTA
Volume: 3.0 mL (minimum: 3.0 mL)

Shipping: Room temperature

Special Instructions: n/a

Testing Schedule(s): Please call

Turnaround Time: 7 days

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For more information, call 416.340.5227 or 1.866.865.5227

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